Isolation and purification ofCeqI endonuclease, an isoschizomer ofEcoRV
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چکیده
منابع مشابه
Isolation and purification of CeqI endonuclease, an isoschizomer of EcoRV.
Ooryrprvcterium equii produces a new restriction endonuclease, an iso-schizemer of EcoKV (1). The bacterial strain does not contain significant levels of other nucleases, so the restriction endonuclease activity can be detected in crude extracts. Ihe enzyme is purified by ammonium sulphate fractionation (50-70 % saturation) and DEAE cellulose chranatography (elutes with 200 mM KC1 in PC buffer)...
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Ptal is a type II restriction endonuclease from the Cyanobacterial strain Phormidium tadzschicicum. This strain was obtained from the Collection of Algal Cultures of Leningrad University. Ptal recognizes the sequence 5'-TCCGGA-3' and cleaves between T and C. The enzyme was purified using the following chromatographic steps: 1) Phosphocelulose, 2) DEAESephadex. The enzyme was free of contaminati...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.3.1334